منابع مشابه
Y-fluorescence of interphase nuclei, especially circulating lymphocytes.
Application of a fluorescence technique for the detection of the human Y chromosome in interphase nuclei indicates that the best results may be obtained from the study of lymphocytes in blood smears. The most satisfactory Y-fluorescence is obtained with the use of buffered quinacrine mustard dihydrochloride. The method can be complemented by other standard techniques to obtain a clear idea of t...
متن کاملDetection of Dna Synthesis in Interphase Nuclei by Fluorescence Microscopy
INTRODUCTION affords their differentiation from other peripheral blood cells . Previous cytological identification of It is often of interest to identify those cells in a replicating cells has employed autoradiography mixed population which are synthesizing DNA . (3-6) . These cells can now be identified by fluoresFor example, division of thymus-dependent lymcence microscopy (7, 8), due to the ...
متن کاملDetection of chromosome aberrations in interphase nuclei using fluorescence in situ hybridization technique.
We report here several experiences of interphase cytogenetics, using fluorescence in situ hybridization (FISH) technique, for the detection of chromosome aberrations. FISH, using alpha satellite specific probes of 18, X, Y chromosomes, was done in interphase nuclei from peripheral blood of patients with Edwards' syndrome, Klinefelter's syndrome and Turner's syndrome with healthy male and female...
متن کاملInterphase fluorescence in situ hybridisation of the X and Y chromosomes in the human eye.
AIM To determine the sex of individual cells in paraffin sections of the human eye by fluorescence in situ hybridisation (FISH) of the X and Y chromosomes. METHODS The authors developed a protocol for FISH of the X and Y chromosomes in paraffin sections of human eyes. RESULTS In all the specimens that had been fixed in 10% formalin and with a fixation time of up to 3 days sex determination ...
متن کاملChromatin associations in Arabidopsis interphase nuclei
The arrangement of chromatin within interphase nuclei seems to be caused by topological constraints and related to gene expression depending on tissue and developmental stage. In yeast and animals it was found that homologous and heterologous chromatin association are required to realize faithful expression and DNA repair. To test whether such associations are present in plants we analyzed Arab...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BMJ
سال: 1971
ISSN: 0959-8138,1468-5833
DOI: 10.1136/bmj.1.5746.461-c